ABSTRACT
Objective:To establish the protein content determination method respectively by Folin-Ciocalteu method and Coomass-ie brilliant blue binding method for mannatide oral solution and compare the results. Methods:The limit of detection, linearity, accu-racy, repeatability, recovery and content of Folin-Ciocalteu method and Coomassie brilliant blue binding method were investigated. Re-sults:As for Folin-Ciocalteu method, the limit of detection was 0. 2 μg, the range of linearity was 0-112. 0 μg(r=0. 9990), the av-erage recovery was 105. 2%(RSD=1. 9%), the RSD of accuracy was less than 1%, and the content result of three batches of samples was 47. 45, 58. 34 and 40. 99 μg·ml-1, respectively. As for Coomassie brilliant blue binding method, the limit of detection was 0. 8 μg, the range of linearity was 0-99. 6 μg(r=0. 9980), the average recovery was 102. 0%(RSD=2. 7%), the RSD of accuracy was less than 1%, and the protein content of samples was not detected out by the method. Conclusion:Folin-Ciocalteu method can be used to determine the protein content in mannatide oral solution reliably and efficiently.
ABSTRACT
Objective:To establish the protein content determination method respectively by Folin-Ciocalteu method and Coomass-ie brilliant blue binding method for mannatide oral solution and compare the results. Methods:The limit of detection, linearity, accu-racy, repeatability, recovery and content of Folin-Ciocalteu method and Coomassie brilliant blue binding method were investigated. Re-sults:As for Folin-Ciocalteu method, the limit of detection was 0. 2 μg, the range of linearity was 0-112. 0 μg(r=0. 9990), the av-erage recovery was 105. 2%(RSD=1. 9%), the RSD of accuracy was less than 1%, and the content result of three batches of samples was 47. 45, 58. 34 and 40. 99 μg·ml-1, respectively. As for Coomassie brilliant blue binding method, the limit of detection was 0. 8 μg, the range of linearity was 0-99. 6 μg(r=0. 9980), the average recovery was 102. 0%(RSD=2. 7%), the RSD of accuracy was less than 1%, and the protein content of samples was not detected out by the method. Conclusion:Folin-Ciocalteu method can be used to determine the protein content in mannatide oral solution reliably and efficiently.